General Cell Based Assays
Meso Scale Discovery U-PLEX Mouse MIP-1 ASY
The Meso Scale Discovery U-PLEX Mouse MIP-1alpha Assay Kit is a multiplex immunoassay that measures mouse macrophage inflammatory protein 1-alpha (MIP-1alpha) in serum plasma and cell culture supernatants The assay uses electrochemiluminescence (ECL) detection technology and provides high sensitivity up to 5 logs of dynamic range and low sample volume requirements This assay kit includes a 96-well plate coated with capture antibodies detection antibodies labeled with an ECL tag calibrators and diluent The assay takes approximately 3 5 hours to run and 10 L of sample is required The kit can test up to 80 samples in duplicate
Indigo Biosciences Human Adrenoceptor Alpha 1D (ADRA1D) All-inclusive Cell-Based Luciferase Reporter Assay Kit, 96-well assay plate format
Eliminate weeks of cell culture while achieving superior sensitivity with reproducible results from this all-inclusive cell-based luciferase reporter assay. INDIGO’s Human Adrenoceptor Alpha 1D reporter assay allows you to quickly screen test samples to quantify functional activity, either agonist or antagonist, they may exert against ADRA1D. Kit includes cryopreserved reporter cells engineered to provide constitutive expression of the receptor, optimized media for use during cell thawing and in diluting test samples, reference agonist, Luciferase Detection Reagent, cell culture-ready assay plate, and detailed assay protocol. INDIGO’s unique Cryomite™ process provides exceptional cell viability post-thaw and INDIGO’s strict QA process means consistent results lot to lot (product quality data available upon request). This kit allows researchers to achieve superior sensitivity, clear, reproducible results, and eliminate weeks of cell culture work.
Eurofins Discoverx PathHunter eXpress OPRD1 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0400E2CP2L)
The PathHunter OPRD1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. DADLE) based activation of OPRD1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged OPRD1 and EA-tagged β-Arrestin-2. Activation of OPRD1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Molecular Devices Fluorescent Assay Kit, cGMP, 96-Well, Competitive Immunoassay
The CatchPoint cGMP Fluorescent Assay Kit is a 96-well competitive immunoassay for quantitative measurement of 3',5'-cyclic guanosine monophosphate (cGMP) and guanylate cyclase activity in biochemical and cell-based assays. The kit provides sensitive fluorescence-based detection and includes pre-coated microplates and optimized reagents for high-throughput workflows.
- 96-well format with a black microplate and clear bottom.
- Includes reagents and buffers for assay setup and detection.
- Reagents sufficient for two 96-well plates (192 reactions at 120 μL).
- Fluorescence detection using Stoplight Red substrate, compatible with common plate readers.
- Typical sensitivity down to ~0.2 nM cGMP, dynamic range up to ~120 nM.
- Store kit components at 4°C; reconstituted working solutions stable for about three weeks at 4°C.
Sigma Aldrich Fine Chemicals Biosciences fatty Acid Uptake Kit suff
fatty acids are critical for many biological processes including energy metabolism and the synthesis of structural cellular components. The facilitated uptake of fatty acids presumably through fatty acid transport proteins is thought to be regulated at multiple levels. The dysregulation of fatty acid uptake may contribute to diseases such as obesity type 2 diabetes and hepatic steatosis.
Electron Microscopy Sciences Flim Sleeve 135 mm/24 exp with Clear open flap
Polyguard Polypropylene film sleeves are the finest archival quality material, and are guaranteed to give you ultimate protection for your most important film negatives. Polyguard is 100% acid free, as well as being free from optical distortion. It is resistant to water, grease, oil, alkalies, and organic solvents. Polyguard is durable, yet pliable and gentle to the film and will not become brittle, crack, or abrade the film.
Type: Clear open flap
Eurofins Discoverx InCELL Hunter eXpress BLK Kinase Binding Assay, 10pl Kit. (96-0044E1CP0L)
The InCELL Hunter eXpress BLK Kinase Binding Assay provides an easy-to-use cell based assay to study small molecule drug candidates that engage with kinases. This kit assesses compound-engagement-based stabilization of BLK via detection of BLK levels. This product uses EFC technology. The cell line included overexpresses BLK target protein fused to ProLabel. In the absence of compound that binds and stabilizes the target, this protein is degraded and no EFC signal is produced. When compound engages and stabilizes the target, EA contained in the detection reagent produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress PTGER3 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0640E2CP0M)
The PathHunter PTGER3 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. prostaglandin E2) based activation of PTGER3 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged PTGER3 and EA-tagged β-Arrestin-2. Activation of PTGER3 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter Human GPR83 β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Human GPR83 β-Arrestin Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure GPR83 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter Human GPR143 β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Human GPR143 β-Arrestin Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure GPR143 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter eXpress PTGIR Total Internalization Assay, 2pl Kit. (93-0838E3CP0M)
The PathHunter PTGIR Total Internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of PTGIR via detection of localization to the early endosome. The included cell line overexpresses ProLink-tagged PTGIR and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the PTGIR receptor to the early endosome leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx cAMP Hunter Teriparatide Bioassay Kit, 10pl. (95-0118Y2-00058)
The cAMP Hunter Teriparatide Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses Teriparatide based activation of PTHR GPCR activity via detection of levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type PTHR, a naturally Gs coupled GPCR. Upon activation of PTHR, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Eurofins Discoverx PathHunter eXpress mGHSR1a CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0605E2MCP2M)
The PathHunter mGHSR1a eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. grehlin) based activation of mGHSR1a GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mGHSR1a and EA-tagged β-Arrestin-2. Activation of mGHSR1a stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Abcam Fumitremorgin C (Tryptoquivaline), Chemosensitizing agent, 250UG
MW 379.5 Da, Purity >98%. Chemosensitizing agent. Potent and selective BCRP inhibitor. Selective AKT inhibitor. Selectively reverses resistance to and potentiates mitoxantrone (ab145668), doxorubicin (ab120629) and topotecan (ab120388) in BCRP-transfected cells. Mycotoxin. Shows antiproliferative effects. Shows tremorgenic effects in vivo. .
The product is subject to the following: Abcam Restricted Use Statement
Cayman Chemical cGAS Stop Solution, 1 mL
cGAS Stop Solution has been tested and formulated to work exclusively with Cayman’s cGAS Inhibitor Screening Assay Kit (Item No. 701930).